Interleukin-6 (IL-6) has important role as pro-inflammatory cytokine. Study of the molecular characteristic is needed before conducting further research, thus can provide insight and understanding regarding the properties and characteristics of IL-6. Therefore, this study aims to provide comprehensive bioinformatics analysis of IL-6 at biomolecular level. Method: Bioinformatic analysis of IL-6 was obtained from several websites that can be accessed freely on internet, such as NCBI, PSIPRED, PROTPARAM, TMHMM, PROTSCALE, PEPTIDECUTTER, PROSITE, NETNGLYC, TARGETP, SIGNALP, and Swissmodel. Result: Human IL-6 gene is located on chromosome 7p15.3. The IL-6 protein is 212amino acids (aa) long, secondary structure is mostly in helical-shaped amino acid form. The protein is unstable, with half time 30 hours. Most of the IL-6 protein is located outside the cell membrane. It has glycosylation site at the 73 and 172 amino acid positions, with domain motif at positions 101-126. The prediction of the target location is mostly in the signal peptide pathway. Predicted cleavage site is between position 27 and 28 of amino acid. Conclusion: bioinformatics analysis is very useful in determining the molecular characteristics of IL-6.